GENETICALLY MODIFIED PLANTS: THE METHOD BIOLISTICO
Sunday, December 13, 2009
Splenda Or Sweet And Low
modified plants genticamente talking about the method biolistico 
or physical
. This method for genetically modified plants is actually an alternative to using a biological method that sees the use of the bacterium Agrobacterium
, 
or physical . This method for genetically modified plants is actually an alternative to using a biological method that sees the use of the bacterium Agrobacterium
especially for many species of plants such as monocots, which have a low susceptibility to infection by this bacterium. Through this method you can insert inside the vegetable plants
exogenous DNA into organelles such as chloroplasts. Biolistico The method includes the introduction of exogenous DNA directly into the genome of plant cells using a vector of transport of particles of inert metal like gold or tungsten. These particles are covered by the genetic construct containing the gene of interest, together with promoter and terminator. The accession of the genetic construct particles is possible through a procedure that favors the precipitation of DNA on the same articelle. The so-coated particles are literally shot, the speed is impressed that pass through cell walls and reach the nucleus. Many of the affected cells die others will suffer the entrance of the particle without being damaged, among them some incorporate the exogenous DNA resulting so processed. The means of propulsion is used like a cannon, in which through an inert gas such as helium, is establishing a high pressure, following the sudden release accelerates the projectile in this way can cross the cell wall to reach the nucleus. The fabrics that we use in this process can be pieces of leaf, cotyledons, calli, immature embryos and even pollen. As in the biological method here using a marker gene for antibiotic resistance or herbicide, which allows the selection of transformed cells.
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